University of Bristol ()

The University of Bristol is among the UK's top research universities, consistently being rated as one of the best UK Biochemistry Departments by the Higher Education Funding Council for England (HEFCE). Substantial investment has meant that the majority of research and teaching laboratories in the School have been refurbished in recent years. In particular, a significant investment has been made in the Wolfson Bioimaging Facility which contains state of the art microscope facilities and also in the Proteomics facility located in the School of Biochemistry. Research in the school is supported by approximately £8million of externally and competitively awarded income per year. There are 20 Professorial, 17 lecturer Senior lecturers/readers and 6 externally funded fellows and 75 research staff (plus 12 research technicians). The School currently has approximately 77 PhD students including 13 BBSRC, 14 Wellcome Trust and 4 MRC. The School hosts the NIHR Blood and Transplant Unit which is composed of researchers from the School of Biochemistry and Bristol Institute of Transfusion Sciences (BITS) located in NHSBT Filton. NHSBT Filton is also the site of the International Blood Group Reference Laboratory IBGRL, NHSBT Filton World Class Blood processing facility and the Stem Cell bank. BITS also runs a MSc course in Transfusion Sciences.

Role and Commitment of key persons (including supervisors)

Dr Ashley Toye is Reader in Cell Biology at UB and is a Principal Investigators for NHSBT. Dr Toye is Director of the NIHR funded Blood and Transplant Research Unit. He has a research active laboratory and will act as PhD Supervisor. Other staff involved include Dr Tim Satchwell who is an experienced postdoc.

Key Research Facilities, Infrastructure and Equipment

Dr Toye’s laboratory is embedded in School of Biochemistry and he also has laboratory space at NHSBT Filton. His laboratory has access to Category 2 tissue culture facilities, cellular imaging via Wolfson Bioimaging laboratory and his own CellR imaging system, flow cytometry and sorting (via flow facility and share of MACSQuant system), and proteomics facilities. Access to IBGRL research monoclonal antibodies and blood bank.

Status of Research Premises

University of Bristol is a dedicated research intensive University. NHSBT Filton houses dedicated R&D research facilities and manufacturing facilities. Dr Toye leads a research active laboratory located at School of Biochemistry and also NHSBT Filton.

Previous Involvement in Research and Training Programmes

Dr Toye has supervised 6 successful PhDs in red blood cell biology or erythropoiesis and also 3 MSc projects. He is a lecturer on the Biochemistry undergraduate course and also the 4 year Biochemistry MSci course. He hosts students for summer research experience and also student undergraduate projects. He is on multiple PhD panels across University progression panels, actively mentor’s students and conducts PhD vivas. He is also very involved in public engagement.

Current Involvement in Research and Training Programmes

Dr Toye is currently supervising 4 PhD students. Dr Toye is also on multiple PhD progression panels and has conducted multiple PhD vivas. He has one EU 2020 ITN RELEVANCE ESR, is work package lead and has hosted other ESRs. He is managing an overall research budget total of approximately £4 Million (2015-2020). His funders include NHSBT R&D, Wellcome Trust, NIHR, Defence Science Technology Laboratory, EU 2020 ITN RELEVANCE and BBSRC.

Relevant Publications and/ or Research/ Innovation Product

• Moura PL, Hawley BR, Mankelow TJ, Griffiths RE, Dobbe JGG, Streekstra GJ, Anstee DJ, Satchwell TJ, Toye AM. Non-muscle Myosin II drives vesicle loss during human reticulocyte maturation. Haematologica. 2018 doi: 10.3324/haematol.2018.199083. • Hawksworth J, Satchwell TJ, Meinders,M, Daniels,D., Regan, F., Thornton,NM., Wilson,MC., Dobbe, JGG., Streeska, GJ., Trakarnsanga, K., Heesom, K., Anstee, DJ., Frayne J.,Toye AM. Enhancement of red blood cell transfusion compatibility using CRISPR-mediated erythroblast gene editing. (2018) EMBO Molecular Medicine. pii: e8454. doi: 10.15252/emmm.201708454 • Pellegrin S, Haydn-Smith KL, Hampton-O'Neil LA, Hawley BR, Heesom KJ, Fermo E, Bianchi P and Toye AM. Transduction with BBF2H7/CREB3L2 upregulates SEC23A protein in erythroblasts and partially corrects the hypo-glycosylation phenotype associated with CDAII. (2018) Br J Haematol. . doi: 10.1111/bjh.15189. • Heideveld E, Hampton-O’Neil LA, Cross, SJ, van Alphen FPJ, van den Biggelaar M, Toye AM and van den Akker E. Glucocorticoids induce differentiation of monocytes towards macrophages that share functional and phenotypical aspects with erythroblastic island macrophages. Haematologica doi: 10.3324/haematol.2017.179341. • Trakarnsanga, K, Griffiths RE, Wilson, MC, Blair A, Satchwell TJ, , Meinders M, Cogan N, Kupzig S, Kurita R, Nakamura Y, Toye AM, Anstee DJ and J Frayne. (2017) First immortalised adult human erythroid line facilitates sustainable and scalable generation of functional red cells. Nature Communications. 8:14750