Institut National de la Santé et de la Recherche Médicale ()

The French National Institute of Health and Medical Research (INSERM) is a public scientific and technological institute and the only French public research institute to focus entirely on human health. Since 2008 INSERM has the responsibility for the strategic, scientific and operational coordination of biomedical research thanks to the scientific quality of its teams and its ability to conduct translational research, from the laboratory to the patient’s bed. INSERM has 318 research units that are set up in university hospitals, cancer research centres and other entities such as Pasteur and Curie Institutes. The UMR_S 1134 unit “Integrated Biology of the Red Cell” is located at the National Institute of Blood Transfusion (INTS) in Paris. Work conducted in this unit focuses on red blood cell biology in normal and pathological conditions. The unit has a long history and strong expertise in red cell membrane proteins exhibiting blood group antigens and filling adhesion, transport or structural function. Presently the UMR_S 1134 established an extended network of physicians in the field of red cell and haematological disorders such as sickle cell disease, myeloproliferative neoplasms, hereditary spherocytosis, Gaucher disease.

Role and Commitment of key persons (including supervisors)

Wassim El Nemer, PhD: group leader and supervisor (30%); Sylvie Cochet, Engineer: research and training (20%); Mickaël Marin, Engineer: research and training (20%); Jean-Philippe Semblat, Engineer: imaging and flow cytometry (10%); Bérengère Koehl, MD PhD: research and patients blood samples and follow-up (10%); ESR to be enrolled by the ITN (100%)

Key Research Facilities, Infrastructure and Equipment

• Microfluidics platform (Fluigent pumps and MIRO High Speed Camera) • Dynamic adhesion platform (ZEISS Axio Observer and Cellix pumps) • Flow cytometry facility (FACSCanto II) • Confocal microscopy (ZEISS LSM 700) • ImageStream – Amnis apparatus (Imagine Institute) • Cell culture facility

Status of Research Premises

Yes, independent academic research premises.

Previous Involvement in Research and Training Programmes

• Supervisor of 4 PhD students at the University of Paris Diderot: Maria A. Lizarralde Iragorri, (Oct 2014 – Sept 2018); Mégane Brusson Sept 2013 – Feb 2017); Maria De Grandis (Sep 2009 – Mar 2013); Pablo Bartolucci (Sep 2007 – Jan 2011) • Supervisor of 3 Post-doctoral fellows: Vicky Chaar (Jan 2008 – Dec 2009); Anna Guadall (Jan 2012 – Dec 2014); Sophie Lefevre (Sept 2013 – Aug 2015) • Since 2000: supervisor of >20 MSc students and teaching of several PhD and MSc courses • Since 2006: Coordinator of a full-week PhD course “Investigating protein-protein interactions” at Paris Diderot/Paris Descartes Universities • Co-chair of the GR-Ex Training Course on Red Blood Cells: Genesis and Pathophysiology (April 23 - April 26, 2014 - Chantilly (Paris), France); (April 18 – April 20, 2018 - Paris, France)

Current Involvement in Research and Training Programmes

• Supervisor of 2 PhD students at the University of Paris Diderot: Sara El Hoss (Sep 2015 – present); Ralfs Buks (Sept 2016 – present: ITN RELEVANCE) • Coordinator of a full-week PhD course “Investigating protein-protein interactions” at Paris Diderot / Paris Descartes Universities • Teaching in PhD and MSc courses

Relevant Publications and/ or Research/ Innovation Product

• Lizarralde Iragorri MA, El Hoss S, Brousse V, Lefevre SD,..., El Nemer W. A microfluidic approach to study the effect of mechanical stress on erythrocytes in sickle cell disease. Lab Chip. 2018 Sep 26;18(19):2975-2984. • Brusson M, De Grandis M, Cochet S...El Nemer W. Impact of hydroxycarbamide and interferon-α on red cell adhesion and membrane protein expression in polycythemia vera. Haematologica. 2018 Jun;103(6):972-981. • El Nemer W, Picot J, et al. A biomimetic microfluidic chip to study the circulation and mechanical retention of red blood cells in the spleen. Am J Hematol. 2015 Apr;90(4):339-45. • De Grandis M, ... El Nemer W. JAK2V617F activates Lu/BCAM-mediated red cell adhesion in polycythemia vera through an EpoR-independent Rap1/Akt pathway. Blood. 2013 Jan 24;121(4):658-65. • Bartolucci P, Chaar V, ...El Nemer W. Decreased sickle red blood cell adhesion to laminin by hydroxyurea is associated with inhibition of Lu/BCAM protein phosphorylation. Blood. 2010 Sep 23;116(12):2152-9.