Centre National de la Recherche Scientifique ()

Centre National de la Recherche Scientifique is a government-funded research organisation under the responsibility of the French Ministry of research. With 33000 persons, 1144 research units spread throughout the country, CNRS carries out research in all scientific fields of knowledge and conducts interdisciplinary programmes, one major objective being to improve knowledge, promote interdisciplinarity, ensure economic and technological development or solve complex societal needs. CNRS is the French largest research organization, and the largest European one in terms of publications. CNRS has been awarded in 2017 HR Excellence in Research (HRS4R). The Station Biologique of Roscoff belongs to Centre National de la recherche Scientifique as well as Sorbonne University Curie, which is the first University in France. The Institute comprise 30 people, with dedicated mission for research and teaching with a high degree of interdisciplinary. The Roscoff Station has a long tradition of hosting scientists and students and now belongs to the ESFRI project EMBRC offering then all core facilities for modern science. Moreover, since National center for Conference of the CNRS-1 is based at Roscoff, the institute is a hotspot for scientific diffusion. The LIPHY (Laboratoire Interdisciplinaire de Physique) is associated to CNRS and University of Grenoble, and counts 70 permanent scientists with high level of interdisciplinarity (Physics/mechanics, applied math. and biology). The LIPHY is implied in many teaching and education programmes at the local and international levels, and is in close interaction with the hospital and the advanced bioscience lab in Grenoble. This offers a privileged environment for carrier developments.

Role and Commitment of key persons (including supervisors)

Stéphane EGEE Assistant Professor Sorbonne University, PhD & habilitation, 50%, supervisor Guillaume BOUYER research Engineer, CNRS, PhD, 30 %, day-to-day training Laurent Pérès research assistant, CNRS 20% , day to day training

Chaouqi MISBAH (Director of Research at CNRS) 50% supervisor, Alexander FARUTIN (research assistant at CNRS) Phd co-supervisor

Key Research Facilities, Infrastructure and Equipment

CNRS1-Station Biologique of Roscoff, offers full facilities to residents. Among them, we have a sequencing facility, associated with full equipments for RT-QPCR (2 Roche system 384 wells, microarrays). A mass spectrometry facility with two mass spectrometers: a Q-ToF 2 (Micromass) coupled with a capillary liquid chromatograph (CapLC system, Waters) and a Voyager-DE STR (Applied Biosystems), associated with 2D-gel electrophoresis equipment. An imaging platform allowing electron microscopy, Cristallography and Bioinformatic core facilities- A JEOL-1400 transmission electron microscope (TEM), fitted with two high-definition cameras (Aurius and UltraScan, Gatan) interfaced with DigitalMicrograph software-Sample holder for cryo-electron microscropy-A JEOL JSM 5200 scanning electron microscope (SEM). Optical microscopy platform offers TCS SP5 AOBS and SP8 Leica confocal interfaced with LAS AF SP5 software (Leica). Beside these core facilities, specifically, our lab offers 3 independent Rigs of patch-clamp allowing studies in all configurations (single channel recording or whole-cell measurements). 2 setup for indirect measurements of membrane potential (cccp methods), possibility to use radioisotopes for fluxes experiments, biochemistry facilities. Moreover, residency facilities are available allowing students or visitors to benefit temporary renting apartments.

Numerical cluster of 300 cores, many GPU machines, and access to national and international computer centers.

Status of Research Premises

CNRS1: independent academic research premises (University). Yes 3 offices, 3 rooms dedicated for patch-clamp experiments, biochemistry and membrane potential measurements. One L2 safety level, for cell culture shared with another group, comprising all facilities CNRS2 acts in the capacity of supporting authority of LBI2M laboratory, which is a joint research unit of Sorbonne Université and CNRS. PhD degrees at LBI2M are awarded by the associated academic entity Sorbonne Université.

Previous Involvement in Research and Training Programmes

CNRS1 Member of the French Laboratory of Excellence “GREx” in charge of training programs Project leader in Professional Bachelor degrees. Former (2010-2018) Project leader in 3rd year of Bachelor degree for supervision of exchange program. Program leader of training FP6 Early Stage Training program called “ESTeam: 9 trainees who complete their PhD in our laboratory in 2010. CNRS2 Leader of the French German doctoral college since 2014 Main organizer of several international schools on blood flow (2009, 2012, 2016, 2017)

Current Involvement in Research and Training Programmes

CNRS1 WP-leader in EU-ITN RELEVANCE CNRS2 French-German doctoral college

Relevant Publications and/ or Research/ Innovation Product

• Rotordam, G.M., Fermo, E., Becker, N., Barcellini, W., Brüggemann, A., Fertig, N., Egée, S., Markus Rapedius, M., Bianchi, P., Kaestner, L. (2018) A novel gain-of-function mutation of Piezo1 is functionally affirmed in red blood cells by high-throughput patch clamp. Haematologica, in press. • Bouyer G, Reininger L, Ramdani G, L DP, Sharma V, Egee S, Langsley G & Lasonder E. (2016). Plasmodium falciparum infection induces dynamic changes in the erythrocyte phospho-proteome. Blood Cells Mol Dis 58, 35-44. • Bouyer, G., Cueff, A., Egée, S., Kmiecik, J.Maksimova, Y., Glogowska, E., Gallagher, P.G., Thomas.S.L.,Erythrocyte peripheral type benzodiazepine receptor/voltage-dependent anion channels are upregulated by Plasmodium falciparum. Blood. 2011 118(8):2305-12. • Zhang et al. ATP Release by Red Blood Cells under Flow: Model and Simulations, Biphys. J. , 115, 22218 (2018). • Shen et al. Blood Crystal: Emergent Order of Red Blood Cells Under Wall-Confined Shear Flow, 120, 268102 (2018) • Farutin et al. Optimal cell transport in straight channels and networks Phys. Rev. Fluid 3, 103603 (2018).